1kvv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1kvv.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1kvv.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1kvv| PDB=1kvv | SCENE= }}
{{STRUCTURE_1kvv| PDB=1kvv | SCENE= }}
-
'''Solution Structure Of Protein SRP19 Of The Archaeoglobus fulgidus Signal Recognition Particle, Minimized Average Structure'''
+
===Solution Structure Of Protein SRP19 Of The Archaeoglobus fulgidus Signal Recognition Particle, Minimized Average Structure===
-
==Overview==
+
<!--
-
Protein SRP19 is an essential RNA-binding component of the signal recognition particle (SRP) in Archaea and Eucarya. A three-dimensional solution structure of the 104 residue SRP19 from the hyperthermophilic archaeon Archaeoglobus fulgidus, designated as Af19, was determined by NMR spectroscopy. Af19 contains three beta-strands, two alpha-helical regions, arranged in a betaalphabetabetaalpha topology, a 3(10) helix, and a disordered C-terminal tail. This fold is similar to the betaalphabetabetaalphabeta RNP motif present in numerous other RNA-binding proteins, which engage their cognate RNAs using conserved sequence motifs present within beta-strands 1 and 3. Mutagenesis studies of human SRP19, however, reveal the major contact sites with SRP RNA reside within loops 1, 3, and 4. These contacts were verified by the crystal structure of human SRP19 complexed to SRP RNA helix 6 reported subsequent to the submission of the manuscript. The crystal structure also reveals that, unlike canonical RNP motifs, SRP19 does not engage specific RNA bases through conserved sequence motifs present within beta-strands 1 and 3. Instead, SRP19 uses residues both within and flanking beta-strand 1 to stabilize the complex through direct and indirect contacts to the phosphate backbone of the tetraloop, leaving the bases of the tetraloop exposed. This, coupled with the fact that SRP19 appears relatively rigid and undergoes only minor changes in structure upon RNA binding, may underlie the molecular basis by which SRP19 functions to initiate SRP assembly.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11916385}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11916385 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11916385}}
==About this Structure==
==About this Structure==
-
1KVV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KVV OCA].
+
1KVV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KVV OCA].
==Reference==
==Reference==
Line 28: Line 32:
[[Category: Zwieb, C.]]
[[Category: Zwieb, C.]]
[[Category: Rna binding protein]]
[[Category: Rna binding protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:13:50 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:06:54 2008''

Revision as of 08:06, 2 July 2008

Image:1kvv.png

Template:STRUCTURE 1kvv

Solution Structure Of Protein SRP19 Of The Archaeoglobus fulgidus Signal Recognition Particle, Minimized Average Structure

Template:ABSTRACT PUBMED 11916385

About this Structure

1KVV is a Single protein structure of sequence from Archaeoglobus fulgidus. Full experimental information is available from OCA.

Reference

Solution structure of protein SRP19 of Archaeoglobus fulgidus signal recognition particle., Pakhomova ON, Deep S, Huang Q, Zwieb C, Hinck AP, J Mol Biol. 2002 Mar 15;317(1):145-58. PMID:11916385

Page seeded by OCA on Wed Jul 2 11:06:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1kvv"
Personal tools