1kwh

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{{STRUCTURE_1kwh| PDB=1kwh | SCENE= }}
{{STRUCTURE_1kwh| PDB=1kwh | SCENE= }}
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'''Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.'''
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===Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.===
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==Overview==
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Sphingomonas sp. A1 possesses a high molecular mass (average 25,700 Da) alginate uptake system mediated by a novel pit-dependent ABC transporter. The X-ray crystallographic structure of AlgQ2 (57,200 Da), an alginate-binding protein in the system, was determined by the multiple isomorphous replacement method and refined at 2.0 A resolution with a final R-factor of 18.3% for 15 to 2.0 A resolution data. The refined structure of AlgQ2 was comprised of 492 amino acid residues, 172 water molecules, and one calcium ion. AlgQ2 was composed of two globular domains with a deep cleft between them, which is expected to be the alginate-binding site. The overall structure is basically similar to that of maltose/maltodextrin-binding protein, except for the presence of an N2-subdomain. The entire calcium ion-binding site is similar to the site in the EF-hand motif, but comprises a ten residue loop. This calcium ion-binding site is about 40 A away from the alginate-binding site.
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(as it appears on PubMed at http://www.pubmed.gov), where 11884143 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11884143}}
==About this Structure==
==About this Structure==
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[[Category: Alginate]]
[[Category: Alginate]]
[[Category: Binding protein]]
[[Category: Binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:15:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:09:30 2008''

Revision as of 08:09, 2 July 2008

Image:1kwh.png

Template:STRUCTURE 1kwh

Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.

Template:ABSTRACT PUBMED 11884143

About this Structure

1KWH is a Single protein structure of sequence from Sphingomonas sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:11884143

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