1kxk

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{{STRUCTURE_1kxk| PDB=1kxk | SCENE= }}
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'''Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron'''
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===Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron===
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==Overview==
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Group II self-splicing introns catalyze autoexcision from precursor RNA transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases initiates via nucleophilic attack at the 5' splice site by the 2' OH of a conserved intron adenosine residue, creating a branched (lariat) intermediate. Here, we describe the crystal structure at 3.0 A resolution of a 70-nucleotide RNA containing the catalytically essential domains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleotide bulged structure around the branch-point adenosine in domain 6.
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(as it appears on PubMed at http://www.pubmed.gov), where 11859154 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11859154}}
==About this Structure==
==About this Structure==
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[[Category: Tetraloop receptor]]
[[Category: Tetraloop receptor]]
[[Category: Two-nucleotide bulge]]
[[Category: Two-nucleotide bulge]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:17:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:13:53 2008''

Revision as of 08:13, 2 July 2008

Image:1kxk.png

Template:STRUCTURE 1kxk

Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron

Template:ABSTRACT PUBMED 11859154

About this Structure

Full crystallographic information is available from OCA.

Reference

Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:11859154

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