1kzh

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{{STRUCTURE_1kzh| PDB=1kzh | SCENE= }}
{{STRUCTURE_1kzh| PDB=1kzh | SCENE= }}
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'''Structure of a pyrophosphate-dependent phosphofructokinase from the Lyme disease spirochete Borrelia burgdorferi'''
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===Structure of a pyrophosphate-dependent phosphofructokinase from the Lyme disease spirochete Borrelia burgdorferi===
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==Overview==
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The structure of the 60 kDa pyrophosphate (PP(i))-dependent phosphofructokinase (PFK) from Borrelia burgdorferi has been solved and refined (R(free) = 0.243) at 2.55 A resolution. The domain structure of eubacterial ATP-dependent PFKs is conserved in B. burgdorferi PFK, and there are three large insertions relative to E. coli PFK, including a helical domain containing a hairpin structure that interacts with the active site. Asp177, conserved in all PP(i) PFKs, negates the binding of the alpha-phosphate group of ATP and likely contacts the essential Mg(2+) cation via a water molecule. Asn181 blocks the binding of the adenine moiety of ATP. Lys203 hydrogen bonds to a sulfate anion that likely mimics PP(i) substrate binding.
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The line below this paragraph, {{ABSTRACT_PUBMED_12015149}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12015149 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12015149}}
==About this Structure==
==About this Structure==
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[[Category: Pyrophosphate]]
[[Category: Pyrophosphate]]
[[Category: Spirochete]]
[[Category: Spirochete]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:21:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:21:45 2008''

Revision as of 08:21, 2 July 2008

Image:1kzh.png

Template:STRUCTURE 1kzh

Structure of a pyrophosphate-dependent phosphofructokinase from the Lyme disease spirochete Borrelia burgdorferi

Template:ABSTRACT PUBMED 12015149

About this Structure

1KZH is a Single protein structure of sequence from Borrelia burgdorferi. Full crystallographic information is available from OCA.

Reference

The structure of a pyrophosphate-dependent phosphofructokinase from the Lyme disease spirochete Borrelia burgdorferi., Moore SA, Ronimus RS, Roberson RS, Morgan HW, Structure. 2002 May;10(5):659-71. PMID:12015149

Page seeded by OCA on Wed Jul 2 11:21:45 2008

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