1l30

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{{STRUCTURE_1l30| PDB=1l30 | SCENE= }}
{{STRUCTURE_1l30| PDB=1l30 | SCENE= }}
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'''REPLACEMENTS OF PRO86 IN PHAGE T4 LYSOZYME EXTEND AN ALPHA-HELIX BUT DO NOT ALTER PROTEIN STABILITY'''
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===REPLACEMENTS OF PRO86 IN PHAGE T4 LYSOZYME EXTEND AN ALPHA-HELIX BUT DO NOT ALTER PROTEIN STABILITY===
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==Overview==
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To investigate the relation between protein stability and the predicted stabilities of individual secondary structural elements, residue Pro86 in an alpha-helix in phage T4 lysozyme was replaced by ten different amino acids. The x-ray crystal structures of seven of the mutant lysozymes were determined at high resolution. In each case, replacement of the proline resulted in the formation of an extended alpha-helix. This involves a large conformational change in residues 81 to 83 and smaller shifts that extend 20 angstroms across the protein surface. Unexpectedly, all ten amino acid substitutions marginally reduce protein thermostability. This insensitivity of stability to the amino acid at position 86 is not simply explained by statistical and thermodynamic criteria for helical propensity. The observed conformational changes illustrate a general mechanism by which proteins can tolerate mutations.
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(as it appears on PubMed at http://www.pubmed.gov), where 3277275 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3277275}}
==About this Structure==
==About this Structure==
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[[Category: Dao-Pin, S.]]
[[Category: Dao-Pin, S.]]
[[Category: Matthews, B W.]]
[[Category: Matthews, B W.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:29:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:35:12 2008''

Revision as of 08:35, 2 July 2008

Image:1l30.png

Template:STRUCTURE 1l30

REPLACEMENTS OF PRO86 IN PHAGE T4 LYSOZYME EXTEND AN ALPHA-HELIX BUT DO NOT ALTER PROTEIN STABILITY

Template:ABSTRACT PUBMED 3277275

About this Structure

1L30 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Replacements of Pro86 in phage T4 lysozyme extend an alpha-helix but do not alter protein stability., Alber T, Bell JA, Sun DP, Nicholson H, Wozniak JA, Cook S, Matthews BW, Science. 1988 Feb 5;239(4840):631-5. PMID:3277275

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