1l3g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1l3g.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1l3g.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1l3g| PDB=1l3g | SCENE= }}
{{STRUCTURE_1l3g| PDB=1l3g | SCENE= }}
-
'''NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae'''
+
===NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae===
-
==Overview==
+
<!--
-
The three-dimensional solution structure of the DNA-binding domain of Mlu-1 box binding protein (Mbp1) has been determined by multidimensional NMR spectroscopy. Mbp1 is a cell cycle transcription factor from Saccharomyces cerevisiae and consists of an N-terminal DNA-binding domain, a series of ankyrin repeats, and a heterodimerization domain at the C-terminus. A set of conformers comprising 19 refined structures was calculated via a molecular dynamics simulated annealing protocol using distance, dihedral angle, and residual dipolar coupling restraints derived from either double or triple resonance NMR experiments. The solution structure consists of a six-stranded beta-sheet segment folded against two pairs of alpha-helices in the topology of the winged helix-turn-helix family of proteins and is in agreement with the X-ray structures. In addition, the solution structure shows that the C-terminal tail region of this domain folds back and makes specific interactions with the N-terminal beta-strand of the protein. This C-terminal region is essential for full DNA-binding activity but appears in the X-ray structure to be disordered. The fold-back structure extends the region of positive electrostatic potential, and this may enhance the nonspecific contribution to binding by favorable electrostatic interactions with the DNA backbone.
+
The line below this paragraph, {{ABSTRACT_PUBMED_12564929}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 12564929 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_12564929}}
==About this Structure==
==About this Structure==
-
1L3G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3G OCA].
+
1L3G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3G OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Mlu 1 cell cycle box binding protein]]
[[Category: Mlu 1 cell cycle box binding protein]]
[[Category: Winged helix-turn-helix protein]]
[[Category: Winged helix-turn-helix protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:29:49 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:37:06 2008''

Revision as of 08:37, 2 July 2008

Image:1l3g.png

Template:STRUCTURE 1l3g

NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae

Template:ABSTRACT PUBMED 12564929

About this Structure

1L3G is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

NMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae., Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN, Biochemistry. 2003 Feb 11;42(5):1266-73. PMID:12564929

Page seeded by OCA on Wed Jul 2 11:37:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1l3g"
Personal tools