1la4

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[[Image:1la4.gif|left|200px]]
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{{STRUCTURE_1la4| PDB=1la4 | SCENE= }}
{{STRUCTURE_1la4| PDB=1la4 | SCENE= }}
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'''Solution Structure of SGTx1'''
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===Solution Structure of SGTx1===
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==Overview==
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SGTx1 is a peptide toxin isolated from the venom of the spider Scodra griseipes that has been shown to inhibit outward K(+) currents in rat cerebellar granule neurons. Although its amino acid sequence is known to be highly (76%) homologous with that of hanatoxin (HaTx), a well-characterized modifier of Kv2.1 channel gating, the structural and functional characteristics of SGTx1 remain largely unknown. Here we describe the NMR solution structure of SGTx1, the mechanism of its interaction with Kv2.1 channels, and its effect on channel activity once bound. The NMR structure of SGTx1 contains a molecular fold closely resembling the "inhibitor cystine knot" of HaTx, which is composed of an antiparallel beta-sheet and four chain reversals stabilized by three disulfide bonds. Functionally, SGTx1 reversibly inhibited K(+) currents in oocytes expressing Kv2.1 channels. Moreover, generation of steady-state activation curves showed that, consistent with other gating modifiers, SGTx1 acted by shifting the activation of the channel to more depolarized voltages. Thus, the surface profile and mechanism of action of SGTx1 are similar to those of HaTx. Still, detailed comparison of SGTx1 with HaTx revealed differences in binding affinity and conformational homogeneity that result from differences in the charge distribution at the binding surface and in the amino acid composition of the respective beta-hairpin structures in the peptides.
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(as it appears on PubMed at http://www.pubmed.gov), where 14744131 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14744131}}
==About this Structure==
==About this Structure==
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1LA4 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LA4 OCA].
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1LA4 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LA4 OCA].
==Reference==
==Reference==
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[[Category: Peptide neurotoxin]]
[[Category: Peptide neurotoxin]]
[[Category: Triple-stranded antiparallel beta-sheet]]
[[Category: Triple-stranded antiparallel beta-sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:42:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 12:05:43 2008''

Revision as of 09:05, 2 July 2008

Image:1la4.png

Template:STRUCTURE 1la4

Solution Structure of SGTx1

Template:ABSTRACT PUBMED 14744131

About this Structure

1LA4 is a Single protein structure. Full experimental information is available from OCA.

Reference

Solution structure and functional characterization of SGTx1, a modifier of Kv2.1 channel gating., Lee CW, Kim S, Roh SH, Endoh H, Kodera Y, Maeda T, Kohno T, Wang JM, Swartz KJ, Kim JI, Biochemistry. 2004 Feb 3;43(4):890-7. PMID:14744131

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