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1ldy

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[[Image:1ldy.gif|left|200px]]
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[[Image:1ldy.png|left|200px]]
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{{STRUCTURE_1ldy| PDB=1ldy | SCENE= }}
{{STRUCTURE_1ldy| PDB=1ldy | SCENE= }}
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'''HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND CYCLOHEXYL FORMAMIDE (CXF)'''
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===HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND CYCLOHEXYL FORMAMIDE (CXF)===
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==Overview==
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Amides are analogs of aldehydes and potent inhibitors of liver alcohol dehydrogenases. They can be used for structural studies and for inhibiting the metabolism of alcohols that form toxic products. We studied N-alkyl amides that bind to the enzyme-NADH complex and act as uncompetitive inhibitors against varied concentrations of ethanol (millimolar Kii values, at pH 8 and 25 degrees C): N-propylacetamide (16), delta-valerolactam (1.6), N-formylpiperidine (0.14), N-isobutylformamide (0.028), N-(cyclohexylmethyl)-formamide (0.011), and N-cyclohexylformamide (0.0087). The lower affinity of delta-valerolactam and N-propylacetamide can be explained by steric hindrance with Phe93 of the enzyme. Replacing Phe93 with Ala in the S48T/F93A mutated enzyme, which resembles the natural alpha-isoenzyme of primates, improved binding of delta-valerolactam by 210-fold. The structures of horse liver enzyme complexed with NADH and N-cyclohexylformamide or N-formylpiperidine were determined by X-ray crystallography at 2.5 A resolution. In both complexes, the carbonyl oxygens of the inhibitors bind to the catalytic zinc and form a hydrogen bond to the hydroxyl group of Ser48 of the enzyme. The six-membered rings bind in overlapping, but rotated, positions that optimize hydrophobic interactions. The binding modes of the unreactive formamides appear to resemble the Michaelis complexes of the analogous substrates, with the re face of the carbonyl carbon suitably positioned to accept a hydrogen from NADH.
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(as it appears on PubMed at http://www.pubmed.gov), where 9132002 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9132002}}
==About this Structure==
==About this Structure==
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[[Category: Formamide]]
[[Category: Formamide]]
[[Category: Nicotinamide coenzyme]]
[[Category: Nicotinamide coenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:49:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 12:50:22 2008''

Revision as of 09:50, 2 July 2008

Image:1ldy.png

Template:STRUCTURE 1ldy

HORSE LIVER ALCOHOL DEHYDROGENASE COMPLEXED TO NADH AND CYCLOHEXYL FORMAMIDE (CXF)

Template:ABSTRACT PUBMED 9132002

About this Structure

1LDY is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.

Reference

Binding of formamides to liver alcohol dehydrogenase., Ramaswamy S, Scholze M, Plapp BV, Biochemistry. 1997 Mar 25;36(12):3522-7. PMID:9132002

Page seeded by OCA on Wed Jul 2 12:50:22 2008

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