1i4w
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(New page: 200px<br /><applet load="1i4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i4w, resolution 2.60Å" /> '''THE CRYSTAL STRUCTUR...)
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Revision as of 14:56, 20 November 2007
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THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION
Overview
Although it is commonly accepted that binding of mitochondrial, transcription factor sc-mtTFB to the mitochondrial RNA polymerase is, required for specific transcription initiation in Saccharomyces, cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution., The protein consists of two domains, an N-terminal alpha/beta-domain and a, smaller domain made up of four alpha-helices. Contrary to previous, predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but, rather is structurally homologous to rRNA methyltransferase ErmC'. This, suggests that sc-mtTFB functions as an RNA-binding protein, an observation, standing in contradiction to the existing model, which proposed a direct, interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the, structure, we propose that the promoter specificity region is located on, the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly, mediates interaction of the core enzyme with the promoter site.
About this Structure
1I4W is a Single protein structure of sequence from Saccharomyces cerevisiae with XE as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:11567089
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