1le8

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{{STRUCTURE_1le8| PDB=1le8 | SCENE= }}
{{STRUCTURE_1le8| PDB=1le8 | SCENE= }}
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'''Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex'''
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===Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex===
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==Overview==
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Triply mutated MATalpha2 protein, alpha2-3A, in which all three major groove-contacting residues are mutated to alanine, is defective in binding DNA alone or in complex with Mcm1 yet binds with MATa1 with near wild-type affinity and specificity. To gain insight into this unexpected behavior, we determined the crystal structure of the a1/alpha2-3A/DNA complex. The structure shows that the triple mutation causes a collapse of the alpha2-3A/DNA interface that results in a reorganized set of alpha2-3A/DNA contacts, thereby enabling the mutant protein to recognize the wild-type DNA sequence. Isothermal titration calorimetry measurements reveal that a much more favorable entropic component stabilizes the a1/alpha2-3A/DNA complex than the alpha2-3A/DNA complex. The combined structural and thermodynamic studies provide an explanation of how partner proteins influence the sequence specificity of a DNA binding protein.
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{{ABSTRACT_PUBMED_12121651}}
==About this Structure==
==About this Structure==
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[[Category: Matalpha2]]
[[Category: Matalpha2]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:50:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 13:16:51 2008''

Revision as of 10:17, 2 July 2008

Image:1le8.png

Template:STRUCTURE 1le8

Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex

Template:ABSTRACT PUBMED 12121651

About this Structure

1LE8 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural and thermodynamic characterization of the DNA binding properties of a triple alanine mutant of MATalpha2., Ke A, Mathias JR, Vershon AK, Wolberger C, Structure. 2002 Jul;10(7):961-71. PMID:12121651

Page seeded by OCA on Wed Jul 2 13:16:51 2008

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