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1lf9

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{{STRUCTURE_1lf9| PDB=1lf9 | SCENE= }}
{{STRUCTURE_1lf9| PDB=1lf9 | SCENE= }}
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'''CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE'''
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===CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE===
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==Overview==
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The first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain.
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(as it appears on PubMed at http://www.pubmed.gov), where 12614608 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12614608}}
==About this Structure==
==About this Structure==
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[[Category: Carbohydrase family gh15]]
[[Category: Carbohydrase family gh15]]
[[Category: Super beta sandwich]]
[[Category: Super beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:52:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 18:43:01 2008''

Revision as of 15:43, 2 July 2008

Image:1lf9.png

Template:STRUCTURE 1lf9

CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE

Template:ABSTRACT PUBMED 12614608

About this Structure

1LF9 is a Single protein structure of sequence from Thermoanaerobacterium thermosaccharolyticum. Full crystallographic information is available from OCA.

Reference

Crystal structure and evolution of a prokaryotic glucoamylase., Aleshin AE, Feng PH, Honzatko RB, Reilly PJ, J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:12614608

Page seeded by OCA on Wed Jul 2 18:43:01 2008

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