1lm1

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{{STRUCTURE_1lm1| PDB=1lm1 | SCENE= }}
{{STRUCTURE_1lm1| PDB=1lm1 | SCENE= }}
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'''Structural studies on the synchronization of catalytic centers in glutamate synthase: native enzyme'''
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===Structural studies on the synchronization of catalytic centers in glutamate synthase: native enzyme===
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==Overview==
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The complex iron-sulfur flavoprotein glutamate synthase (GltS) plays a prominent role in ammonia assimilation in bacteria, yeasts, and plants. GltS catalyzes the formation of two molecules of l-glutamate from 2-oxoglutarate and l-glutamine via intramolecular channeling of ammonia. GltS has the impressive ability of synchronizing its distinct catalytic centers to avoid wasteful consumption of l-glutamine. We have determined the crystal structure of the ferredoxin-dependent GltS in several ligation and redox states. The structures reveal the crucial elements in the synchronization between the glutaminase site and the 2-iminoglutarate reduction site. The structural data combined with the catalytic properties of GltS indicate that binding of ferredoxin and 2-oxoglutarate to the FMN-binding domain of GltS induce a conformational change in the loop connecting the two catalytic centers. The rearrangement induces a shift in the catalytic elements of the amidotransferase domain, such that it becomes activated. This machinery, over a distance of more than 30 A, controls the ability of the enzyme to bind and hydrolyze the ammonia-donating substrate l-glutamine.
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(as it appears on PubMed at http://www.pubmed.gov), where 11967268 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11967268}}
==About this Structure==
==About this Structure==
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[[Category: Channeling]]
[[Category: Channeling]]
[[Category: Glutamate synthase]]
[[Category: Glutamate synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:03:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:24:11 2008''

Revision as of 18:24, 2 July 2008

Image:1lm1.png

Template:STRUCTURE 1lm1

Structural studies on the synchronization of catalytic centers in glutamate synthase: native enzyme

Template:ABSTRACT PUBMED 11967268

About this Structure

1LM1 is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.

Reference

Structural studies on the synchronization of catalytic centers in glutamate synthase., van den Heuvel RH, Ferrari D, Bossi RT, Ravasio S, Curti B, Vanoni MA, Florencio FJ, Mattevi A, J Biol Chem. 2002 Jul 5;277(27):24579-83. Epub 2002 Apr 19. PMID:11967268

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