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1lmk

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{{STRUCTURE_1lmk| PDB=1lmk | SCENE= }}
{{STRUCTURE_1lmk| PDB=1lmk | SCENE= }}
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'''THE STRUCTURE OF A BIVALENT DIABODY'''
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===THE STRUCTURE OF A BIVALENT DIABODY===
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==Overview==
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BACKGROUND: Diabodies are dimeric antibody fragments. In each polypeptide, a heavy-chain variable domain (VH) is linked to a light-chain variable domain (VL) but unlike single-chain Fv fragments, each antigen-binding site is formed by pairing of one VH and one VL domain from the two different polypeptides. Diabodies thus have two antigen-binding sites, and can be bispecific. Direct structural evidence is lacking for the connections and dimeric interactions between the two polypeptides of the diabody. RESULTS: The 2.6 A resolution structure has been determined for a bivalent diabody with a flexible five-residue polypeptide linker between the (amino-terminal) VH and (carboxy-terminal) VL domains. The asymmetric unit of the crystal consists of four polypeptides comprising two diabodies; for one of these polypeptides the linker can be traced between the VH and VL domains. Within each diabody the two associated VH and VL domains make back-to-back interactions through the VH domains, and there is an extensive VL-VL interface between the two diabodies in the asymmetric unit. CONCLUSIONS: The structure of the diabody is very similar to that which had been predicted by molecular modelling. Diabodies directed against cell-surface antigens should be capable of bringing together two cells, such as in cell-targeted therapy, because the two antigen-binding sites of the diabody are at opposite ends of the molecule and separated by approximately 65 A.
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The line below this paragraph, {{ABSTRACT_PUBMED_7704531}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 7704531 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7704531}}
==About this Structure==
==About this Structure==
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[[Category: Williams, R L.]]
[[Category: Williams, R L.]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:03:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:25:48 2008''

Revision as of 18:25, 2 July 2008

Image:1lmk.png

Template:STRUCTURE 1lmk

THE STRUCTURE OF A BIVALENT DIABODY

Template:ABSTRACT PUBMED 7704531

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of a diabody, a bivalent antibody fragment., Perisic O, Webb PA, Holliger P, Winter G, Williams RL, Structure. 1994 Dec 15;2(12):1217-26. PMID:7704531

Page seeded by OCA on Wed Jul 2 21:25:48 2008

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