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| | {{STRUCTURE_1lmk| PDB=1lmk | SCENE= }} | | {{STRUCTURE_1lmk| PDB=1lmk | SCENE= }} |
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| - | '''THE STRUCTURE OF A BIVALENT DIABODY'''
| + | ===THE STRUCTURE OF A BIVALENT DIABODY=== |
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| - | ==Overview==
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| - | BACKGROUND: Diabodies are dimeric antibody fragments. In each polypeptide, a heavy-chain variable domain (VH) is linked to a light-chain variable domain (VL) but unlike single-chain Fv fragments, each antigen-binding site is formed by pairing of one VH and one VL domain from the two different polypeptides. Diabodies thus have two antigen-binding sites, and can be bispecific. Direct structural evidence is lacking for the connections and dimeric interactions between the two polypeptides of the diabody. RESULTS: The 2.6 A resolution structure has been determined for a bivalent diabody with a flexible five-residue polypeptide linker between the (amino-terminal) VH and (carboxy-terminal) VL domains. The asymmetric unit of the crystal consists of four polypeptides comprising two diabodies; for one of these polypeptides the linker can be traced between the VH and VL domains. Within each diabody the two associated VH and VL domains make back-to-back interactions through the VH domains, and there is an extensive VL-VL interface between the two diabodies in the asymmetric unit. CONCLUSIONS: The structure of the diabody is very similar to that which had been predicted by molecular modelling. Diabodies directed against cell-surface antigens should be capable of bringing together two cells, such as in cell-targeted therapy, because the two antigen-binding sites of the diabody are at opposite ends of the molecule and separated by approximately 65 A.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_7704531}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7704531 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_7704531}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Williams, R L.]] | | [[Category: Williams, R L.]] |
| | [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:03:58 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:25:48 2008'' |
Revision as of 18:25, 2 July 2008
Template:STRUCTURE 1lmk
THE STRUCTURE OF A BIVALENT DIABODY
Template:ABSTRACT PUBMED 7704531
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of a diabody, a bivalent antibody fragment., Perisic O, Webb PA, Holliger P, Winter G, Williams RL, Structure. 1994 Dec 15;2(12):1217-26. PMID:7704531
Page seeded by OCA on Wed Jul 2 21:25:48 2008
