1i8y

From Proteopedia

---

Revision as of 15:02, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1i8y Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SEMI-AUTOMATIC STRUCTURE DETERMINATION OF THE CG1 3-30 PEPTIDE BASED ON ARIA

Overview

Carp granulins are members of an emerging class of proteins with a, sequence motif encoding a parallel stack of two to four beta-hairpins. The, carp granulin-1 protein forms a stack of four beta-hairpins, whereas its, amino-terminal fragment appears to adopt a very stable stack of two, beta-hairpins in solution. Here we determined a refined three-dimensional, structure of this peptide fragment to examine potential conformational, changes compared with the full-length protein. The structures were, calculated with both a traditional method and a fast semiautomated method, using ambiguous NMR distance restraints. The resulting sets of structures, are very similar and show that a well-defined stack of two beta-hairpins, is retained in the peptide. Conformational rearrangements compensating the, loss of the carboxy-terminal subdomain of the native protein are, restricted to the carboxy-terminal end of the peptide, the turn connecting, the two beta-hairpins, and the Tyr(21) and Tyr(25) aromatic side chains., Further removal of the Val(1) and Ile(2) residues, which are part of the, first beta-hairpin and components of two major hydrophobic clusters in the, two beta-hairpin structure, results in the loss of the first beta-hairpin., The second beta-hairpin, which is closely associated with the first, retains a similar but somewhat less stable conformation. The invariable, presence of the second beta-hairpin and the dependence of its stability on, the first beta-hairpin suggest that the stack of two beta-hairpins may be, an evolutionary conserved and autonomous folding unit. In addition, the, high conformational stability makes the stack of two beta-hairpins an, attractive scaffold for the development of peptide-based drug candidates.

About this Structure

1I8Y is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Solution structures of a 30-residue amino-terminal domain of the carp granulin-1 protein and its amino-terminally truncated 3-30 subfragment: implications for the conformational stability of the stack of two beta-hairpins., Vranken WF, James S, Bennett HP, Ni F, Proteins. 2002 Apr 1;47(1):14-24. PMID:11870861

Page seeded by OCA on Tue Nov 20 17:10:06 2007