1i9d

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Revision as of 15:04, 20 November 2007

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spinqualitylabelsall models 1i9d, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

ARSENATE REDUCTASE FROM E. COLI

Overview

BACKGROUND: In Escherichia coli bearing the plasmid R773, resistance to, arsenite, arsenate, antimonite, and tellurite is conferred by the arsRDABC, plasmid operon that codes for an ATP-dependent anion pump. The product of, the arsC gene, arsenate reductase (ArsC), is required to efficiently, catalyze the reduction of arsenate to arsenite prior to extrusion., RESULTS: Here, we report the first X-ray crystal structures of ArsC at, 1.65 A and of ArsC complexed with arsenate and arsenite at 1.26 A, resolution. The overall fold is unique. The native structure shows sulfate, and sulfite ions binding in the active site as analogs of arsenate and, arsenite. The covalent adduct of arsenate with Cys-12 in the active site, of ArsC, which was analyzed in a difference map, shows tetrahedral, geometry with a sulfur-arsenic distance of 2.18 A. However, the, corresponding adduct with arsenite binds as a hitherto unseen, thiarsahydroxy adduct. Finally, the number of bound waters (385) in this, highly ordered crystal structure approaches twice the number expected at, this resolution for a structure of 138 ordered residues. CONCLUSIONS:, Structural information from the adduct of ArsC with its substrate, (arsenate) and with its product (arsenite) together with functional, information from mutational and biochemical studies on ArsC suggest a, plausible mechanism for the reaction. The exceptionally well-defined water, structure indicates that this crystal system has precise long-range order, within the crystal and that the upper limit for the number of bound waters, in crystal structures is underestimated by the structures in the Protein, Data Bank.

About this Structure

1I9D is a Single protein structure of sequence from Escherichia coli with SO4, SO3 and CS as ligands. Full crystallographic information is available from OCA.

Reference

Insights into the structure, solvation, and mechanism of ArsC arsenate reductase, a novel arsenic detoxification enzyme., Martin P, DeMel S, Shi J, Gladysheva T, Gatti DL, Rosen BP, Edwards BF, Structure. 2001 Nov;9(11):1071-81. PMID:11709171

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