1lr6

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{{STRUCTURE_1lr6| PDB=1lr6 | SCENE= }}
{{STRUCTURE_1lr6| PDB=1lr6 | SCENE= }}
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'''Crystal structure of V45Y mutant of cytochrome b5'''
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===Crystal structure of V45Y mutant of cytochrome b5===
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==Overview==
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Val45 is a highly conserved residue and a component of the heme-pocket wall of cytochrome b(5). The crystal structures of cytochrome b(5) mutants V45E and V45Y have been determined at high resolution. Their overall structures were very similar to that of the wild-type protein. However, Val45 of the wild-type protein points towards the heme, but the large side chains of both Glu45 and Tyr45 of the mutants point towards the solvent. A channel is thus opened and the hydrophobicity of the heme pocket is decreased. The rotation of the porphyrin ring and the conformational change of the axial ligand His39 in the V45Y mutant indicate that the microenvironment of the heme is disturbed because of the mutation. The binding constants and the electron-transfer rates between cytochrome b(5) and cytochrome c decrease owing to the mutation, which can be accounted for by molecular modeling: the inter-iron distances increase in order to eliminate the unreasonably close contacts resulting from the large volumes of the mutated side chains. The influence of the mutations on the redox potentials and protein stability is also discussed. The structures of seven mutants of cytochrome b(5) are compared with each other and the effects of these mutations on the protein properties and functions are summarized.
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(as it appears on PubMed at http://www.pubmed.gov), where 12136141 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12136141}}
==About this Structure==
==About this Structure==
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[[Category: Mutant v45y]]
[[Category: Mutant v45y]]
[[Category: Trypsin-solubilized fragment]]
[[Category: Trypsin-solubilized fragment]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:12:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:51:40 2008''

Revision as of 18:51, 2 July 2008

Image:1lr6.png

Template:STRUCTURE 1lr6

Crystal structure of V45Y mutant of cytochrome b5

Template:ABSTRACT PUBMED 12136141

About this Structure

1LR6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structures of V45E and V45Y mutants and structure comparison of a variety of cytochrome b5 mutants., Gan JH, Wu J, Wang ZQ, Wang YH, Huang ZX, Xia ZX, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1298-306. Epub 2002, Jul 20. PMID:12136141

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