1lrr

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[[Image:1lrr.gif|left|200px]]
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{{Seed}}
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[[Image:1lrr.png|left|200px]]
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{{STRUCTURE_1lrr| PDB=1lrr | SCENE= }}
{{STRUCTURE_1lrr| PDB=1lrr | SCENE= }}
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'''CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA'''
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===CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA===
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==Overview==
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The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation.
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The line below this paragraph, {{ABSTRACT_PUBMED_12379844}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12379844 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12379844}}
==About this Structure==
==About this Structure==
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[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Replication]]
[[Category: Replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:13:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:54:53 2008''

Revision as of 18:54, 2 July 2008

Image:1lrr.png

Template:STRUCTURE 1lrr

CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA

Template:ABSTRACT PUBMED 12379844

About this Structure

1LRR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex., Guarne A, Zhao Q, Ghirlando R, Yang W, Nat Struct Biol. 2002 Nov;9(11):839-43. PMID:12379844

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