1ia0
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(New page: 200px<br /><applet load="1ia0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ia0, resolution 15.Å" /> '''KIF1A HEAD-MICROTUBUL...)
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Revision as of 15:05, 20 November 2007
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KIF1A HEAD-MICROTUBULE COMPLEX STRUCTURE IN ATP-FORM
Overview
Kinesin motors are specialized enzymes that use hydrolysis of ATP to, generate force and movement along their cellular tracks, the microtubules., Although numerous biochemical and biophysical studies have accumulated, much data that link microtubule-assisted ATP hydrolysis to kinesin motion, the structural view of kinesin movement remains unclear. This study of the, monomeric kinesin motor KIF1A combines X-ray crystallography and, cryo-electron microscopy, and allows analysis of force-generating, conformational changes at atomic resolution. The motor is revealed in its, two functionally critical states-complexed with ADP and with a, non-hydrolysable analogue of ATP. The conformational change observed, between the ADP-bound and the ATP-like structures of the KIF1A catalytic, core is modular, extends to all kinesins and is similar to the, conformational change used by myosin motors and G proteins. Docking of the, ADP-bound and ATP-like crystallographic models of KIF1A into the, corresponding cryo-electron microscopy maps suggests a rationale for the, plus-end directional bias associated with the kinesin catalytic core.
About this Structure
1IA0 is a Protein complex structure of sequences from Mus musculus and Sus scrofa with MG, GTP, GDP, TXL and ACP as ligands. Full crystallographic information is available from OCA.
Reference
Switch-based mechanism of kinesin motors., Kikkawa M, Sablin EP, Okada Y, Yajima H, Fletterick RJ, Hirokawa N, Nature. 2001 May 24;411(6836):439-45. PMID:11373668
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