1iaw
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1iaw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iaw, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...)
Next diff →
Revision as of 15:06, 20 November 2007
|
CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA
Overview
NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase, activities when a Lys residue is substituted for Leu 43. The NaeI-DNA, structure demonstrates that each of the two domains of NaeI recognizes one, molecule of DNA duplex. DNA recognition induces dramatic rearrangements:, narrowing the binding site of the Topo domain 16 A to grip DNA, widening, that of the Endo domain 8 A to encircle and bend DNA 45 degrees for, cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA, structure presents the first example of novel recognition of two copies of, one DNA sequence by two different amino acid sequences and two different, structural motifs in one polypeptide.
About this Structure
1IAW is a Single protein structure of sequence from Lechevalieria aerocolonigenes. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
Reference
Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement., Huai Q, Colandene JD, Topal MD, Ke H, Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254
Page seeded by OCA on Tue Nov 20 17:13:34 2007
