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1luc

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{{STRUCTURE_1luc| PDB=1luc | SCENE= }}
{{STRUCTURE_1luc| PDB=1luc | SCENE= }}
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'''BACTERIAL LUCIFERASE'''
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===BACTERIAL LUCIFERASE===
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==Overview==
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Bacterial luciferase is a flavin monooxygenase that catalyzes the oxidation of a long-chain aldehyde and releases energy in the form of visible light. A new crystal form of luciferase cloned from Vibrio harveyi has been grown under low-salt concentrations, which diffract x-rays beyond 1.5-A resolution. The x-ray structure of bacterial luciferase has been refined to a conventional R-factor of 18.2% for all recorded synchrotron data between 30.0 and 1.50-A resolution. Bacterial luciferase is an alpha-beta heterodimer, and the individual subunits fold into a single domain (beta/alpha)8 barrel. The high resolution structure reveals a non-prolyl cis peptide bond that forms between Ala74 and Ala75 in the alpha subunit near the putative active site. This cis peptide bond may have functional significance for creating a cavity at the active site. Bacterial luciferase employs reduced flavin as a substrate rather than a cofactor. The structure presented was determined in the absence of substrates. A comparison of the structural similarities between luciferase and a nonfluorescent flavoprotein, which is expressed in the lux operon of one genus of bioluminescent bacteria, suggests that the two proteins originated from a common ancestor. However, the flavin binding sites of the nonfluorescent protein are likely not representative of the flavin binding site on luciferase. The structure presented here will furnish a detailed molecular model for all bacterial luciferases.
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{{ABSTRACT_PUBMED_8703001}}
==About this Structure==
==About this Structure==
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[[Category: Flavoprotein]]
[[Category: Flavoprotein]]
[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:17:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:15:58 2008''

Revision as of 19:16, 2 July 2008

Image:1luc.png

Template:STRUCTURE 1luc

BACTERIAL LUCIFERASE

Template:ABSTRACT PUBMED 8703001

About this Structure

1LUC is a Protein complex structure of sequences from Vibrio harveyi. Full crystallographic information is available from OCA.

Reference

The 1.5-A resolution crystal structure of bacterial luciferase in low salt conditions., Fisher AJ, Thompson TB, Thoden JB, Baldwin TO, Rayment I, J Biol Chem. 1996 Sep 6;271(36):21956-68. PMID:8703001

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