1lvo

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{{STRUCTURE_1lvo| PDB=1lvo | SCENE= }}
{{STRUCTURE_1lvo| PDB=1lvo | SCENE= }}
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'''Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain'''
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===Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain===
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==Overview==
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The key enzyme in coronavirus polyprotein processing is the viral main proteinase, M(pro), a protein with extremely low sequence similarity to other viral and cellular proteinases. Here, the crystal structure of the 33.1 kDa transmissible gastroenteritis (corona)virus M(pro) is reported. The structure was refined to 1.96 A resolution and revealed three dimers in the asymmetric unit. The mutual arrangement of the protomers in each of the dimers suggests that M(pro) self-processing occurs in trans. The active site, comprised of Cys144 and His41, is part of a chymotrypsin-like fold that is connected by a 16 residue loop to an extra domain featuring a novel alpha-helical fold. Molecular modelling and mutagenesis data implicate the loop in substrate binding and elucidate S1 and S2 subsites suitable to accommodate the side chains of the P1 glutamine and P2 leucine residues of M(pro) substrates. Interactions involving the N-terminus and the alpha-helical domain stabilize the loop in the orientation required for trans-cleavage activity. The study illustrates that RNA viruses have evolved unprecedented variations of the classical chymotrypsin fold.
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(as it appears on PubMed at http://www.pubmed.gov), where 12093723 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12093723}}
==About this Structure==
==About this Structure==
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[[Category: Cysteine histidine dyad]]
[[Category: Cysteine histidine dyad]]
[[Category: Proteinase]]
[[Category: Proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:20:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:30:33 2008''

Revision as of 19:30, 2 July 2008

Image:1lvo.png

Template:STRUCTURE 1lvo

Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain

Template:ABSTRACT PUBMED 12093723

About this Structure

1LVO is a Single protein structure of sequence from Transmissible gastroenteritis virus. Full crystallographic information is available from OCA.

Reference

Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain., Anand K, Palm GJ, Mesters JR, Siddell SG, Ziebuhr J, Hilgenfeld R, EMBO J. 2002 Jul 1;21(13):3213-24. PMID:12093723

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