1ibi
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(New page: 200px<br /><applet load="1ibi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ibi" /> '''QUAIL CYSTEINE AND GLYCINE-RICH PROTEIN, NMR...)
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Revision as of 15:07, 20 November 2007
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QUAIL CYSTEINE AND GLYCINE-RICH PROTEIN, NMR, 15 MINIMIZED MODEL STRUCTURES
Overview
The solution structure of quail CRP2(LIM2) was significantly improved by, using an increased number of NOE constraints obtained from a, 13C,15N-labeled protein sample and by applying a recently developed, triple-resonance cross-correlated relaxation experiment for the, determination of the backbone dihedral angle psi. Additionally, the, relative orientation of the 15N(i)-1HN(i) dipole and the 13CO(i) CSA, tensor, which is related to both backbone angles phi and psi, was probed, by nitrogen-carbonyl multiple-quantum relaxation and used as an additional, constraint for the refinement of the local geometry of the, metal-coordination sites in CRP2(LIM2). The backbone dynamics of residues, located in the folded part of CRP2(LIM2) have been characterized by, proton-detected 13C'(i-1)-15N(i) and 15N(i)-1HN(i) multiple-quantum, relaxation, respectively. We show that regions having cross-correlated, time modulation of backbone isotropic chemical shifts on the millisecond, to microsecond time scale correlate with residues that are structurally, altered in the mutant protein CRP2(LIM2)R122A (disruption of the CCHC, zinc-finger stabilizing side-chain hydrogen bond) and that these residues, are part of an extended hydrogen-bonding network connecting the two, zinc-binding sites. This indicates the presence of long-range collective, motions in the two zinc-binding subdomains. The conformational plasticity, of the LIM domain may be of functional relevance for this important, protein recognition motif.
About this Structure
1IBI is a Single protein structure of sequence from Coturnix japonica with ZN as ligand. Full crystallographic information is available from OCA.
Reference
Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains., Schuler W, Kloiber K, Matt T, Bister K, Konrat R, Biochemistry. 2001 Aug 14;40(32):9596-604. PMID:11583159
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