1lwl

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{{STRUCTURE_1lwl| PDB=1lwl | SCENE= }}
{{STRUCTURE_1lwl| PDB=1lwl | SCENE= }}
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'''Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)'''
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===Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)===
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==Overview==
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We have synthesized two luminescent probes (D-4-Ad and D-8-Ad) that target cytochrome P450cam. D-4-Ad luminescence is quenched by Forster energy transfer upon binding (Kd = 0.83 muM) but is restored when the probe is displaced from the active site by camphor. In contrast, D-8-Ad (Kd approximately 0.02 muM) is not displaced from the enzyme, even in the presence of a large excess of camphor. The 2.2 A resolution crystal structure of the D-8-Ad:P450cam complex reveals extensive hydrophobic contacts between the probe and the enzyme, which result from the conformational flexibility of the B', F, and G helices. Probes with properties similar to those of D-4-Ad potentially could be useful for screening P450 inhibitors.
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(as it appears on PubMed at http://www.pubmed.gov), where 12197708 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12197708}}
==About this Structure==
==About this Structure==
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[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Substrate-binding]]
[[Category: Substrate-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:22:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:42:51 2008''

Revision as of 19:42, 2 July 2008

Image:1lwl.png

Template:STRUCTURE 1lwl

Crystal Structure of Cytochrome P450-cam with a Fluorescent Probe D-8-Ad (Adamantane-1-carboxylic acid-5-dimethylamino-naphthalene-1-sulfonylamino-octyl-amide)

Template:ABSTRACT PUBMED 12197708

About this Structure

1LWL is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Fluorescent probes for cytochrome p450 structural characterization and inhibitor screening., Dunn AR, Hays AM, Goodin DB, Stout CD, Chiu R, Winkler JR, Gray HB, J Am Chem Soc. 2002 Sep 4;124(35):10254-5. PMID:12197708

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