1lwt

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{{STRUCTURE_1lwt| PDB=1lwt | SCENE= }}
{{STRUCTURE_1lwt| PDB=1lwt | SCENE= }}
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'''Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)'''
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===Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)===
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==Overview==
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The first X-ray structures of an intein-DNA complex, that of the two-domain homing endonuclease PI-SceI bound to its 36-base pair DNA substrate, have been determined in the presence and absence of Ca(2+). The DNA shows an asymmetric bending pattern, with a major 50 degree bend in the endonuclease domain and a minor 22 degree bend in the splicing domain region. Distortions of the DNA bound to the endonuclease domain cause the insertion of the two cleavage sites in the catalytic center. DNA binding induces changes in the protein conformation. The two overlapping non-identical active sites in the endonucleolytic center contain two Ca(+2) ions that coordinate to the catalytic Asp residues. Structure analysis indicates that the top strand may be cleaved first.
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The line below this paragraph, {{ABSTRACT_PUBMED_12219083}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12219083 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12219083}}
==About this Structure==
==About this Structure==
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[[Category: Intein]]
[[Category: Intein]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:22:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:43:41 2008''

Revision as of 19:43, 2 July 2008

Image:1lwt.png

Template:STRUCTURE 1lwt

Crystal structure of the intein homing endonuclease PI-SceI bound to its substrate DNA (Ca2+ free)

Template:ABSTRACT PUBMED 12219083

About this Structure

1LWT is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence., Moure CM, Gimble FS, Quiocho FA, Nat Struct Biol. 2002 Oct;9(10):764-70. PMID:12219083

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