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1m00

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[[Image:1m00.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1m00| PDB=1m00 | SCENE= }}
{{STRUCTURE_1m00| PDB=1m00 | SCENE= }}
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'''Rat neuronal NOS heme domain with N-butyl-N'-hydroxyguanidine bound'''
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===Rat neuronal NOS heme domain with N-butyl-N'-hydroxyguanidine bound===
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==Overview==
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A series of N-alkyl-N'-hydroxyguanidine compounds have recently been characterized as non-amino acid substrates for all three nitric oxide synthase (NOS) isoforms which mimic NO formation from N(omega)-hydroxy-L-arginine. Crystal structures of the nNOS heme domain complexed with either N-isopropyl-N'-hydroxyguanidine or N-butyl-N'-hydroxyguanidine reveal two different binding modes in the substrate binding pocket. The binding mode of the latter is consistent with that observed for the substrate N(omega)-hydroxy-L-arginine bound in the nNOS active site. However, the former binds to nNOS in an unexpected fashion, thus providing new insights into the mechanism on how the hydroxyguanidine moiety leads to NO formation. Structural features of substrate binding support the view that the OH-substituted guanidine nitrogen, instead of the hydroxyl oxygen, is the source of hydrogen supplied to the active ferric-superoxy species for the second step of the NOS catalytic reaction.
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The line below this paragraph, {{ABSTRACT_PUBMED_12437343}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12437343 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12437343}}
==About this Structure==
==About this Structure==
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[[Category: Nitric oxide synthase]]
[[Category: Nitric oxide synthase]]
[[Category: Oxydoreductase]]
[[Category: Oxydoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:28:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 22:54:45 2008''

Revision as of 19:54, 2 July 2008

Image:1m00.png

Template:STRUCTURE 1m00

Rat neuronal NOS heme domain with N-butyl-N'-hydroxyguanidine bound

Template:ABSTRACT PUBMED 12437343

About this Structure

1M00 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

The novel binding mode of N-alkyl-N'-hydroxyguanidine to neuronal nitric oxide synthase provides mechanistic insights into NO biosynthesis., Li H, Shimizu H, Flinspach M, Jamal J, Yang W, Xian M, Cai T, Wen EZ, Jia Q, Wang PG, Poulos TL, Biochemistry. 2002 Nov 26;41(47):13868-75. PMID:12437343

Page seeded by OCA on Wed Jul 2 22:54:45 2008

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