This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1idf
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1idf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1idf, resolution 2.5Å" /> '''ISOCITRATE DEHYDROGEN...)
Next diff →
Revision as of 15:11, 20 November 2007
|
ISOCITRATE DEHYDROGENASE K230M MUTANT APO ENZYME
Overview
Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution, were used to solve the structures of two sequential intermediates formed, during the catalytic actions of isocitrate dehydrogenase. Both, intermediates are distinct from the enzyme-substrate and enzyme-product, complexes. Mutation of key catalytic residues changed the rate determining, steps so that protein and substrate intermediates within the overall, reaction pathway could be visualized.
About this Structure
1IDF is a Single protein structure of sequence from Escherichia coli. Active as Isocitrate dehydrogenase (NADP(+)), with EC number 1.1.1.42 Full crystallographic information is available from OCA.
Reference
Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase., Bolduc JM, Dyer DH, Scott WG, Singer P, Sweet RM, Koshland DE Jr, Stoddard BL, Science. 1995 Jun 2;268(5215):1312-8. PMID:7761851
Page seeded by OCA on Tue Nov 20 17:18:42 2007
