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| - | [[Image:1m3e.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1m3e.png|left|200px]] |
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| | {{STRUCTURE_1m3e| PDB=1m3e | SCENE= }} | | {{STRUCTURE_1m3e| PDB=1m3e | SCENE= }} |
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| - | '''Succinyl-COA:3-ketoacid COA transferase from pig heart (selenomethionine)'''
| + | ===Succinyl-COA:3-ketoacid COA transferase from pig heart (selenomethionine)=== |
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| - | ==Overview==
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| - | Ketoacidosis affects patients who are deficient in the enzyme activity of succinyl-CoA:3-ketoacid CoA transferase (SCOT), since SCOT catalyses the activation of acetoacetate in the metabolism of ketone bodies. Thus far, structure/function analysis of the mammalian enzyme has been predicted based on the three-dimensional structure of a CoA transferase determined from an anaerobic bacterium that utilizes its enzyme for glutamate fermentation. To better interpret clinical data, we have determined the structure of a mammalian CoA transferase from pig heart by X-ray crystallography to 2.5 A resolution. Instrumental to the structure determination were selenomethionine substitution and the use of argon during purification and crystallization. Although pig heart SCOT adopts an alpha/beta protein fold, resembling the overall fold of the bacterial CoA transferase, several loops near the active site of pig heart SCOT follow different paths than the corresponding loops in the bacterial enzyme, accounting for differences in substrate specificities. Two missense mutations found associated with SCOT of ketoacidosis patients were mapped to a location in the structure that might disrupt the stabilization of the amino-terminal strand and thereby interfere with the proper folding of the protein into a functional enzyme.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_12463743}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12463743 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12463743}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Wolodko, W T.]] | | [[Category: Wolodko, W T.]] |
| | [[Category: Alpha/beta protein]] | | [[Category: Alpha/beta protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:35:11 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:07:57 2008'' |
Revision as of 20:07, 2 July 2008
Template:STRUCTURE 1m3e
Succinyl-COA:3-ketoacid COA transferase from pig heart (selenomethionine)
Template:ABSTRACT PUBMED 12463743
About this Structure
1M3E is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Structure of the mammalian CoA transferase from pig heart., Bateman KS, Brownie ER, Wolodko WT, Fraser ME, Biochemistry. 2002 Dec 10;41(49):14455-62. PMID:12463743
Page seeded by OCA on Wed Jul 2 23:07:57 2008
