1iee

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spinqualitylabelsall models 1iee, resolution 0.94Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF TETRAGONAL HEN EGG WHITE LYSOZYME AT 0.94 A FROM CRYSTALS GROWN BY THE COUNTER-DIFFUSION METHOD

Overview

Very high quality crystals of tetragonal hen egg-white lysozyme were grown, in the Advanced Protein Crystallization Facility (APCF) on board the Space, Shuttle using a modified free-interface diffusion (FID) reactor designed, ad hoc to have a longer diffusion path. This design allows the performance, of true counter-diffusion experiments. Crystals were obtained under the, classical chemical conditions defined 50 y ago with NaCl as a, crystallizing agent and acetate pH 4.5 as a buffer. Counter-diffusion, crystallization allows a "physical" instead of chemical optimization of, growth conditions: indeed, this method screens for the best, supersaturation conditions in a single trial and yields crystals of very, high quality. A complete diffraction data set was collected at atomic, resolution from one of these crystals using synchrotron radiation at the, DESY-EMBL beamlines. The overall R(merge) on intensities in the resolution, range 31-0.94 A was 5.2% and the data were 98.9% complete. Refinement was, carried out with the programs CNS and SHELX97 to a final crystallographic, R factor of 12.26% for 72 390 reflections. A mean standard uncertainty in, the atomic positions of 0.024 A was estimated from inversion of blocked, least-squares matrices. 22 side chains show alternate conformations and, the loop 59-75 adopts in the same crystal packing two conformations that, were observed for either triclinic or tetragonal lysozyme in previous, high-resolution studies. In addition to 255 water molecules, the, crystallizing agent (one hexacoordinated sodium ion and five chloride, anions) participates in the ordered lysozyme hydration shell.

About this Structure

1IEE is a Single protein structure of sequence from Gallus gallus with NA and CL as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.

Reference

Structure of tetragonal hen egg-white lysozyme at 0.94 A from crystals grown by the counter-diffusion method., Sauter C, Otalora F, Gavira JA, Vidal O, Giege R, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1119-26. Epub 2001, Jul 23. PMID:11468395

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