1m9l

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{{STRUCTURE_1m9l| PDB=1m9l | SCENE= }}
{{STRUCTURE_1m9l| PDB=1m9l | SCENE= }}
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'''Relaxation-based Refined Structure Of Chlamydomonas Outer Arm Dynein Light Chain 1'''
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===Relaxation-based Refined Structure Of Chlamydomonas Outer Arm Dynein Light Chain 1===
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==Overview==
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The light chain 1 (LC1) polypeptide is a member of the leucine-rich repeat protein family and binds at or near the ATP hydrolytic site within the motor domain of the gamma heavy chain from Chlamydomonas outer arm dynein. It consists of an N-terminal helix, a central barrel formed from six leucine-rich repeats that fold as beta beta alpha units, and a C-terminal helical domain that protrudes from the main axis defined by the leucine-rich repeats. Interaction with the gamma heavy chain is likely mediated through a hydrophobic patch on the larger beta sheet face, and the C-terminal region is predicted to insert into the dynein ATP hydrolytic site. Here we have used 1H-15N heteronuclear relaxation measurements obtained at 500 and 600 MHz to refine and validate the LC1 solution structure. In this refined structure, the C-terminal helix is significantly reoriented by more than 20 degrees as compared to the control and provides a more precise understanding of the potential regulatory role of this domain. We also employed the refined structure to perform a dynamic analysis of LC1 using the 600 MHz data set. These results, which were cross validated using the 500 MHz data set, strongly support identification of the predicted LC1 binding surfaces and provide additional insight into the interaction mechanisms of leucine-rich repeat proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 12515539 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12515539}}
==About this Structure==
==About this Structure==
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1M9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9L OCA].
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1M9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M9L OCA].
==Reference==
==Reference==
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[[Category: Structural refinement]]
[[Category: Structural refinement]]
[[Category: Structure from molmol]]
[[Category: Structure from molmol]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:47:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:30:07 2008''

Revision as of 20:30, 2 July 2008

Image:1m9l.png

Template:STRUCTURE 1m9l

Relaxation-based Refined Structure Of Chlamydomonas Outer Arm Dynein Light Chain 1

Template:ABSTRACT PUBMED 12515539

About this Structure

1M9L is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full experimental information is available from OCA.

Reference

Relaxation-based structure refinement and backbone molecular dynamics of the dynein motor domain-associated light chain., Wu H, Blackledge M, Maciejewski MW, Mullen GP, King SM, Biochemistry. 2003 Jan 14;42(1):57-71. PMID:12515539

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