1mac

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{{STRUCTURE_1mac| PDB=1mac | SCENE= }}
{{STRUCTURE_1mac| PDB=1mac | SCENE= }}
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'''CRYSTAL STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF BACILLUS MACERANS ENDO-1,3-1,4-BETA-GLUCANASE'''
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===CRYSTAL STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF BACILLUS MACERANS ENDO-1,3-1,4-BETA-GLUCANASE===
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==Overview==
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In beta-glucans those beta-1,4 glycosidic bonds which are adjacent to beta-1,3 bonds are cleaved by endo-1,3-1,4-beta-glucanases (beta-glucanases). Here, the relationship between structure and activity of the beta-glucanase of Bacillus macerans is studied by x-ray crystallography and site-directed mutagenesis of active site residues. Crystal structure analysis at 2.3-A resolution reveals a jelly-roll protein structure with a deep active site channel harboring the amino acid residues Trp101, Glu103, Asp105, and Glu107 as in the hybrid Bacillus beta-glucanase H(A16-M) (Keitel, T., Simon, O., Borriss, R., and Heinemann, U. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 5287-5291). Different mutant proteins with substitutions in these residues are generated by site-directed mutagenesis, isolated, and characterized. Compared with the wild-type enzyme their activity is reduced to less than 1%. Several mutants with isosteric substitutions in Glu103 and Glu107 are completely inactive, suggesting a direct role of these residues in glycosyl bond hydrolysis. The kinetic properties of mutant beta-glucanases and the crystal structure of the wild-type enzyme are consistent with a mechanism where Glu103 and Glu107 are the catalytic amino acid residues responsible for cleavage of the beta-1,4 glycosidic bond within the substrate molecule.
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{{ABSTRACT_PUBMED_7852389}}
==About this Structure==
==About this Structure==
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[[Category: Hahn, M.]]
[[Category: Hahn, M.]]
[[Category: Heinemann, U.]]
[[Category: Heinemann, U.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:33:17 2008''

Revision as of 20:33, 2 July 2008

Image:1mac.png

Template:STRUCTURE 1mac

CRYSTAL STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF BACILLUS MACERANS ENDO-1,3-1,4-BETA-GLUCANASE

Template:ABSTRACT PUBMED 7852389

About this Structure

1MAC is a Single protein structure of sequence from Paenibacillus macerans. Full crystallographic information is available from OCA.

Reference

Crystal structure and site-directed mutagenesis of Bacillus macerans endo-1,3-1,4-beta-glucanase., Hahn M, Olsen O, Politz O, Borriss R, Heinemann U, J Biol Chem. 1995 Feb 17;270(7):3081-8. PMID:7852389

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