1mb9

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{{STRUCTURE_1mb9| PDB=1mb9 | SCENE= }}
{{STRUCTURE_1mb9| PDB=1mb9 | SCENE= }}
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'''BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP'''
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===BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP===
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==Overview==
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The catalytic cycle of the ATP/Mg(2+)-dependent enzyme beta-lactam synthetase (beta-LS) from Streptomyces clavuligerus has been observed through a series of x-ray crystallographic snapshots. Chemistry is initiated by the ordered binding of ATP/Mg(2+) and N(2)-(carboxyethyl)-l-arginine (CEA) to the apoenzyme. The apo and ATP/Mg(2+) structures described here, along with the previously described CEA.alpha,beta-methyleneadenosine 5'-triphosphate (CEA.AMP-CPP)/Mg(2+) structure, illuminate changes in active site geometry that favor adenylation. In addition, an acyladenylate intermediate has been trapped. The substrate analog N(2)-(carboxymethyl)-l-arginine (CMA) was adenylated by ATP in the crystal and represents a close structural analog of the previously proposed CEA-adenylate intermediate. Finally, the structure of the ternary product complex deoxyguanidinoproclavaminic acid (DGPC).AMP/PP(i)/Mg(2+) has been determined. The CMA-AMP/PP(i)/Mg(2+) and DGPC.AMP/PP(i)/Mg(2+) structures reveal interactions in the active site that facilitate beta-lactam formation. All of the ATP-bound structures differ from the previously described CEA.AMP-CPP/Mg(2+) structure in that two Mg(2+) ions are found in the active sites. These Mg(2+) ions play critical roles in both the adenylation and beta-lactamization reactions.
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{{ABSTRACT_PUBMED_12409610}}
==About this Structure==
==About this Structure==
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[[Category: Clavulanic acid]]
[[Category: Clavulanic acid]]
[[Category: Deoxyguanidinoproclavaminic acid]]
[[Category: Deoxyguanidinoproclavaminic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:36:42 2008''

Revision as of 20:36, 2 July 2008

Image:1mb9.png

Template:STRUCTURE 1mb9

BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP

Template:ABSTRACT PUBMED 12409610

About this Structure

1MB9 is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.

Reference

The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots., Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14752-7. Epub 2002 Oct 30. PMID:12409610

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