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1mf2

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{{STRUCTURE_1mf2| PDB=1mf2 | SCENE= }}
{{STRUCTURE_1mf2| PDB=1mf2 | SCENE= }}
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'''ANTI HIV1 PROTEASE FAB COMPLEX'''
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===ANTI HIV1 PROTEASE FAB COMPLEX===
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==Overview==
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F11.2.32, a monoclonal antibody raised against HIV-1 protease (Kd = 5 nM), which inhibits proteolytic activity of the enzyme (K(inh) = 35(+/-3)nM), has been studied by crystallographic methods. The three-dimensional structure of the complex between the Fab fragment and a synthetic peptide, spanning residues 36 to 46 of the protease, has been determined at 2.2 A resolution, and that of the Fab in the free state has been determined at 2.6 A resolution. The refined model of the complex reveals ten well-ordered residues of the peptide (P36 to P45) bound in a hydrophobic cavity at the centre of the antigen-binding site. The peptide adopts a beta hairpin-like structure in which residues P38 to P42 form a type II beta-turn conformation. An intermolecular antiparallel beta-sheet is formed between the peptide and the CDR3-H loop of the antibody; additional polar interactions occur between main-chain atoms of the peptide and hydroxyl groups from tyrosine residues protruding from CDR1-L and CDR3-H. Three water molecules, located at the antigen-antibody interface, mediate polar interactions between the peptide and the most buried hypervariable loops, CDR3-L and CDR1-H. A comparison between the free and complexed Fab fragments shows that significant conformational changes occur in the long hypervariable regions, CDR1-L and CDR3-H, upon binding the peptide. The conformation of the bound peptide, which shows no overall structural similarity to the corresponding segment in HIV-1 protease, suggests that F11.2.32 might inhibit proteolysis by distorting the native structure of the enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 9150407 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9150407}}
==About this Structure==
==About this Structure==
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[[Category: Hiv1 protease]]
[[Category: Hiv1 protease]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:57:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:49:47 2008''

Revision as of 20:49, 2 July 2008

Image:1mf2.png

Template:STRUCTURE 1mf2

ANTI HIV1 PROTEASE FAB COMPLEX

Template:ABSTRACT PUBMED 9150407

About this Structure

1MF2 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of an Fab-peptide complex: structural basis of HIV-1 protease inhibition by a monoclonal antibody., Lescar J, Stouracova R, Riottot MM, Chitarra V, Brynda J, Fabry M, Horejsi M, Sedlacek J, Bentley GA, J Mol Biol. 1997 Apr 18;267(5):1207-22. PMID:9150407

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