2by3

From Proteopedia

---

Revision as of 17:58, 29 October 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 2by3, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

IS RADIATION DAMAGE DEPENDENT ON THE DOSE-RATE USED DURING MACROMOLECULAR CRYSTALLOGRAPHY DATA COLLECTION

Overview

This paper focuses on the radiation-damage effects when applying the same, total X-ray dose to protein crystals at different dose rates. These, experiments have been performed on both a selenomethionated protein and on, bovine trypsin using dose rates that span nearly two orders of magnitude., The results show no clear dose-rate effect on the global indicators of, radiation damage, but a small measurable dose-rate effect could be found, when studying specific radiation damage. It is hypothesized that this, observed dose-rate effect relates to differences in the steady-state, free-radical concentration.

About this Structure

2BY3 is a [Single protein] structure of sequence from [Deinococcus radiodurans] with GLC, MG, TRS, TRE and BME as [ligands]. Active as [[1]], with EC number [3.2.1.1]. Full crystallographic information is available from [OCA].

Reference

Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?, Leiros HK, Timmins J, Ravelli RB, McSweeney SM, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):125-32. Epub 2006, Jan 18. PMID:16421442

Page seeded by OCA on Mon Oct 29 20:02:57 2007