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1mi6

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[[Image:1mi6.gif|left|200px]]
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[[Image:1mi6.png|left|200px]]
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{{STRUCTURE_1mi6| PDB=1mi6 | SCENE= }}
{{STRUCTURE_1mi6| PDB=1mi6 | SCENE= }}
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'''Docking of the modified RF2 X-ray structure into the Low Resolution Cryo-EM map of RF2 E.coli 70S Ribosome'''
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===Docking of the modified RF2 X-ray structure into the Low Resolution Cryo-EM map of RF2 E.coli 70S Ribosome===
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==Overview==
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Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC.
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The line below this paragraph, {{ABSTRACT_PUBMED_12511960}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12511960 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12511960}}
==About this Structure==
==About this Structure==
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[[Category: Conformational change]]
[[Category: Conformational change]]
[[Category: Rf2,release complex]]
[[Category: Rf2,release complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:05:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:01:40 2008''

Revision as of 21:01, 2 July 2008

Image:1mi6.png

Template:STRUCTURE 1mi6

Docking of the modified RF2 X-ray structure into the Low Resolution Cryo-EM map of RF2 E.coli 70S Ribosome

Template:ABSTRACT PUBMED 12511960

About this Structure

1MI6 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A cryo-electron microscopic study of ribosome-bound termination factor RF2., Rawat UB, Zavialov AV, Sengupta J, Valle M, Grassucci RA, Linde J, Vestergaard B, Ehrenberg M, Frank J, Nature. 2003 Jan 2;421(6918):87-90. PMID:12511960

Page seeded by OCA on Thu Jul 3 00:01:40 2008

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