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1mjp

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[[Image:1mjp.gif|left|200px]]
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{{STRUCTURE_1mjp| PDB=1mjp | SCENE= }}
{{STRUCTURE_1mjp| PDB=1mjp | SCENE= }}
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'''METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR'''
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===METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR===
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==Overview==
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BACKGROUND: The methionine repressor, MetJ, represses the transcription of genes involved in methionine biosynthesis by binding to arrays of two to five adjacent copies of an eight base-pair 'metbox' sequence. Naturally occurring operators differ from the consensus sequence to a greater extent as the number of metboxes increases. MetJ, while accommodating this sequence variation in natural operators, is very sensitive to particular base changes, even where bases are not directly contacted in the crystal structure of a complex formed between the repressor and consensus operator. RESULTS: Here we report the high-resolution structure of a MetJ mutant, Q44K, bound to the consensus operator sequence (Q44Kwt19) and two related sequences containing mutations at sites believed to be important for indirect readout at non-contacted bases. The overall structure of the Q44Kwt19 complex is very similar to the wild-type complex, but there are small variations in sugar-phosphate backbone conformation and direct contacts to the DNA bases. The mutant complexes show a mixture of direct and indirect readout of sequence variations, with differences in direct contacts and DNA conformation. CONCLUSIONS: Comparison of the wild-type and mutant repressor-operator complexes shows that the repressor makes sufficiently strong interactions with the sugar-phosphate backbone to accommodate some variation in operator sequence with minor changes in direct bases contacts. The reduction in repressor affinity for the two mutant repressor complexes can be partially attributed to a loss in direct contacts to the DNA. In one case, however, the replacement of a flexible TA base-step leads to an unfavourable DNA conformation that reduces the stability of the repressor-operator complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 10986458 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10986458}}
==About this Structure==
==About this Structure==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:13:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:07:52 2008''

Revision as of 21:07, 2 July 2008

Image:1mjp.png

Template:STRUCTURE 1mjp

METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR

Template:ABSTRACT PUBMED 10986458

About this Structure

1MJP is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Direct and indirect readout in mutant Met repressor-operator complexes., Garvie CW, Phillips SE, Structure. 2000 Sep 15;8(9):905-14. PMID:10986458

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