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1mm2

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{{STRUCTURE_1mm2| PDB=1mm2 | SCENE= }}
{{STRUCTURE_1mm2| PDB=1mm2 | SCENE= }}
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'''Solution structure of the 2nd PHD domain from Mi2b'''
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===Solution structure of the 2nd PHD domain from Mi2b===
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==Overview==
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The design of proteins with tailored functions remains a relatively elusive goal. Small size, a well-defined structure, and the ability to maintain structural integrity despite multiple mutations are all desirable properties for such designer proteins. Many zinc binding domains fit this description. We determined the structure of a PHD finger from the transcriptional cofactor Mi2beta and investigated the suitability of this domain as a scaffold for presenting selected binding functions. The two flexible loops in the structure were mutated extensively by either substitution or expansion, without affecting the overall fold of the domain. A binding site for the corepressor CtBP2 was also grafted onto the domain, creating a new PHD domain that can specifically bind CtBP2 both in vitro and in the context of a eukaryotic cell nucleus. These results represent a step toward designing new regulatory proteins for modulating aberrant gene expression in vivo.
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(as it appears on PubMed at http://www.pubmed.gov), where 12842043 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12842043}}
==About this Structure==
==About this Structure==
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1MM2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM2 OCA].
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1MM2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM2 OCA].
==Reference==
==Reference==
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[[Category: Protein scaffold]]
[[Category: Protein scaffold]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:24:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:18:22 2008''

Revision as of 21:18, 2 July 2008

Image:1mm2.png

Template:STRUCTURE 1mm2

Solution structure of the 2nd PHD domain from Mi2b

Template:ABSTRACT PUBMED 12842043

About this Structure

1MM2 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Engineering a protein scaffold from a PHD finger., Kwan AH, Gell DA, Verger A, Crossley M, Matthews JM, Mackay JP, Structure. 2003 Jul;11(7):803-13. PMID:12842043

Page seeded by OCA on Thu Jul 3 00:18:22 2008

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