1moo

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{{STRUCTURE_1moo| PDB=1moo | SCENE= }}
{{STRUCTURE_1moo| PDB=1moo | SCENE= }}
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'''Site Specific Mutant (H64A) of Human Carbonic Anhydrase II at high resolution'''
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===Site Specific Mutant (H64A) of Human Carbonic Anhydrase II at high resolution===
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==Overview==
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Using synchrotron radiation and a CCD detector, X-ray data have been collected at 100 K for the His64Ala mutant of human carbonic anhydrase II complexed with 4-methylimidazole (4-MI) to a maximal 1.05 A resolution, allowing full anisotropic least-squares refinement. The refined model has a conventional R factor of 15.7% for all reflections. The C(alpha) coordinates of the model presented here have an r.m.s. deviation of 0.10 A relative to the previously determined structure at 1.6 A resolution. Several amino-acid residues (six of the 255 observed) have been identified with multiple rotamer side-chain conformations. C, N and O atoms can be differentiated with selective electron-density map contouring. The estimated standard deviations for all main-chain non-H atom bond lengths and angles are 0.013 and 0.030 A, respectively, based on unrestrained full-matrix least-squares refinement. This structure gives detailed information about the tetrahedrally arranged zinc ion coordinated by three histidine N atoms (His94 N(epsilon 2), His96 N(epsilon2) and His119 N(delta1)) and a water/hydroxide, the multiple binding sites of the proton chemical rescue molecule 4-MI and the solvent networks linking the zinc-bound water/hydroxide and 4-MI molecules. This structure presents the highest resolution structure of a carbonic anhydrase isozyme so far determined and adds to the understanding of proton-transfer processes.
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(as it appears on PubMed at http://www.pubmed.gov), where 12499545 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12499545}}
==About this Structure==
==About this Structure==
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[[Category: Twisted beta sheet]]
[[Category: Twisted beta sheet]]
[[Category: Zinc metalloenzyme]]
[[Category: Zinc metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:31:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:35:09 2008''

Revision as of 21:35, 2 July 2008

Image:1moo.png

Template:STRUCTURE 1moo

Site Specific Mutant (H64A) of Human Carbonic Anhydrase II at high resolution

Template:ABSTRACT PUBMED 12499545

About this Structure

1MOO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The refined atomic structure of carbonic anhydrase II at 1.05 A resolution: implications of chemical rescue of proton transfer., Duda D, Govindasamy L, Agbandje-McKenna M, Tu C, Silverman DN, McKenna R, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):93-104. Epub 2002, Dec 19. PMID:12499545

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