1mow

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{{STRUCTURE_1mow| PDB=1mow | SCENE= }}
{{STRUCTURE_1mow| PDB=1mow | SCENE= }}
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'''E-DreI'''
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===E-DreI===
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==Overview==
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We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.
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{{ABSTRACT_PUBMED_12419232}}
==About this Structure==
==About this Structure==
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[[Category: Laglidadg]]
[[Category: Laglidadg]]
[[Category: Structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:36:52 2008''

Revision as of 21:36, 2 July 2008

Image:1mow.png

Template:STRUCTURE 1mow

E-DreI

Template:ABSTRACT PUBMED 12419232

About this Structure

1MOW is a Single protein structure of sequence from Desulfurococcus mobilis and chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

Design, activity, and structure of a highly specific artificial endonuclease., Chevalier BS, Kortemme T, Chadsey MS, Baker D, Monnat RJ, Stoddard BL, Mol Cell. 2002 Oct;10(4):895-905. PMID:12419232

Page seeded by OCA on Thu Jul 3 00:36:52 2008

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