1mp1

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[[Image:1mp1.gif|left|200px]]
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{{STRUCTURE_1mp1| PDB=1mp1 | SCENE= }}
{{STRUCTURE_1mp1| PDB=1mp1 | SCENE= }}
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'''Solution structure of the PWI motif from SRm160'''
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===Solution structure of the PWI motif from SRm160===
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==Overview==
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The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.
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(as it appears on PubMed at http://www.pubmed.gov), where 12600940 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12600940}}
==About this Structure==
==About this Structure==
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1MP1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA].
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1MP1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MP1 OCA].
==Reference==
==Reference==
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[[Category: Szymczyna, B R.]]
[[Category: Szymczyna, B R.]]
[[Category: Four helix bundle]]
[[Category: Four helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:32:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:38:07 2008''

Revision as of 21:38, 2 July 2008

Image:1mp1.png

Template:STRUCTURE 1mp1

Solution structure of the PWI motif from SRm160

Template:ABSTRACT PUBMED 12600940

About this Structure

1MP1 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing., Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ, Genes Dev. 2003 Feb 15;17(4):461-75. PMID:12600940

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