From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:1mpz.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:1mpz.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1mpz| PDB=1mpz | SCENE= }} | | {{STRUCTURE_1mpz| PDB=1mpz | SCENE= }} |
| | | | |
| - | '''NMR solution structure of native Viperidae lebetina obtusa protein'''
| + | ===NMR solution structure of native Viperidae lebetina obtusa protein=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The solution structure of obtustatin, a novel non-RGD disintegrin of 41 residues isolated from Vipera lebetina obtusa venom, and a potent and selective inhibitor of the adhesion of integrin alpha(1)beta(1) to collagen IV, has been determined by two-dimensional nuclear magnetic resonance. Almost the whole set of chemical shifts for 1H, 13C and 15N were assigned at natural abundance from 2D homonuclear and heteronuclear 500 MHz, 600 MHz and 800 MHz spectra at pH 3.0 recorded at 298 K and 303 K. Final structural constraints consisted of 302 non-redundant NOE (95 long-range, 60 medium, 91 sequential and 56 intra-residue), four disulfide bond distances, five chi1 dihedral angles and four hydrogen bonds. The 20 conformers with lowest total energy had no NOE violations greater than 0.35A or dihedral angle violations greater than 12 degrees. The average root-mean-square deviation (RMSD) for backbone atoms of all residues among the 20 conformers was 1.1A and 0.6A for the 29 best-defined residues. Obtustatin lacks any secondary structure. Compared to all known disintegrin structures in which the RGD motif is located at the apex of an 11 residue hairpin loop, the active KTS tripeptide of obtustatin is oriented towards a side of its nine residue integrin-binding loop. The C-terminal tail is near to the active loop, and these two structural elements display the largest atomic displacements due to local conformational disorder. Double cross-peaks for W20, Y28 and H27 in the aromatic region of TOCSY spectra, local RMSD values for these residues, and positive cross-peaks in a ROESY spectrum (600 MHz, 100 ms mixing time), suggest that these residues act as a hinge allowing for the overall flexibility of the entire integrin-binding loop. These distinct structural features, along with its different electrostatic surface potential in relation to other known disintegrins, may confer to obtustatin its reported alpha(1)beta(1) integrin inhibitory selectivity. | + | The line below this paragraph, {{ABSTRACT_PUBMED_12742023}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12742023 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_12742023}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| - | 1MPZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vipera_lebetina_obtusa Vipera lebetina obtusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPZ OCA]. | + | 1MPZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vipera_lebetina_obtusa Vipera lebetina obtusa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MPZ OCA]. |
| | | | |
| | ==Reference== | | ==Reference== |
| Line 28: |
Line 32: |
| | [[Category: Moreno-Murciano, M P.]] | | [[Category: Moreno-Murciano, M P.]] |
| | [[Category: Disintegrin]] | | [[Category: Disintegrin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:34:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:47:38 2008'' |
Revision as of 21:47, 2 July 2008
Template:STRUCTURE 1mpz
NMR solution structure of native Viperidae lebetina obtusa protein
Template:ABSTRACT PUBMED 12742023
About this Structure
1MPZ is a Single protein structure of sequence from Vipera lebetina obtusa. Full experimental information is available from OCA.
Reference
NMR solution structure of the non-RGD disintegrin obtustatin., Paz Moreno-Murciano M, Monleon D, Marcinkiewicz C, Calvete JJ, Celda B, J Mol Biol. 2003 May 23;329(1):135-45. PMID:12742023
Page seeded by OCA on Thu Jul 3 00:47:38 2008
