1mq1

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{{STRUCTURE_1mq1| PDB=1mq1 | SCENE= }}
{{STRUCTURE_1mq1| PDB=1mq1 | SCENE= }}
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'''Ca2+-S100B-TRTK-12 complex'''
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===Ca2+-S100B-TRTK-12 complex===
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==Overview==
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The Alzheimer-linked neural protein S100B is a signaling molecule shown to control the assembly of intermediate filament proteins in a calcium-sensitive manner. Upon binding calcium, a conformational change occurs in S100B exposing a hydrophobic surface for target protein interactions. The synthetic peptide TRTK-12 (TRTKIDWNKILS), derived from random bacteriophage library screening, bears sequence similarity to several intermediate filament proteins and has the highest calcium-dependent affinity of any target molecule for S100B to date (K(d) &lt;1 microm). In this work, the three-dimensional structure of the Ca(2+)-S100B-TRTK-12 complex has been determined by NMR spectroscopy. The structure reveals an extended, contiguous hydrophobic surface is formed on Ca(2+)-S100B for target interaction. The TRTK-12 peptide adopts a coiled structure that fits into a portion of this surface, anchored at Trp(7), and interacts with multiple hydrophobic contacts in helices III and IV of Ca(2+)-S100B. This interaction is strikingly different from the alpha-helical structures found for other S100 target peptides. By using the TRTK-12 interaction as a guide, in combination with other available S100 target structures, a recognition site on helix I is identified that may act in concert with the TRTK-12-binding site from helices III and IV. This would provide a larger, more complex site to interact with full-length target proteins and would account for the promiscuity observed for S100B target protein interactions.
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(as it appears on PubMed at http://www.pubmed.gov), where 12480931 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12480931}}
==About this Structure==
==About this Structure==
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1MQ1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQ1 OCA].
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1MQ1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MQ1 OCA].
==Reference==
==Reference==
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[[Category: Ef-hand]]
[[Category: Ef-hand]]
[[Category: Protein-peptide complex]]
[[Category: Protein-peptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:34:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 00:48:50 2008''

Revision as of 21:48, 2 July 2008

Image:1mq1.png

Template:STRUCTURE 1mq1

Ca2+-S100B-TRTK-12 complex

Template:ABSTRACT PUBMED 12480931

About this Structure

1MQ1 is a Protein complex structure of sequences from Homo sapiens. Full experimental information is available from OCA.

Reference

A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex., McClintock KA, Shaw GS, J Biol Chem. 2003 Feb 21;278(8):6251-7. Epub 2002 Dec 11. PMID:12480931

Page seeded by OCA on Thu Jul 3 00:48:50 2008

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