We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

3hdd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3hdd.gif|left|200px]]
+
{{Seed}}
 +
[[Image:3hdd.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3hdd| PDB=3hdd | SCENE= }}
{{STRUCTURE_3hdd| PDB=3hdd | SCENE= }}
-
'''ENGRAILED HOMEODOMAIN DNA COMPLEX'''
+
===ENGRAILED HOMEODOMAIN DNA COMPLEX===
-
==Overview==
+
<!--
-
We report the 2.2 A resolution structure of the Drosophila engrailed homeodomain bound to its optimal DNA site. The original 2.8 A resolution structure of this complex provided the first detailed three-dimensional view of how homeodomains recognize DNA, and has served as the basis for biochemical studies, structural studies and molecular modeling. Our refined structure confirms the principal conclusions of the original structure, but provides important new details about the recognition interface. Biochemical and NMR studies of other homeodomains had led to the notion that Gln50 was an especially important determinant of specificity. However, our refined structure shows that this side-chain makes no direct hydrogen bonds to the DNA. The structure does reveal an extensive network of ordered water molecules which mediate contacts to several bases and phosphates (including contacts from Gln50), and our model provides a basis for detailed comparison with the structure of an engrailed Q50K altered-specificity variant. Comparing our structure with the crystal structure of the free protein confirms that the N and C termini of the homeodomain become ordered upon DNA-binding. However, we also find that several key DNA contact residues in the recognition helix have the same conformation in the free and bound protein, and that several water molecules also are "preorganized" to contact the DNA. Our structure helps provide a more complete basis for the detailed analysis of homeodomain-DNA interactions.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9813123}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9813123 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9813123}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Rould, M A.]]
[[Category: Rould, M A.]]
[[Category: Dna binding]]
[[Category: Dna binding]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:03:39 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 12:48:39 2008''

Revision as of 09:48, 3 July 2008

Image:3hdd.png

Template:STRUCTURE 3hdd

ENGRAILED HOMEODOMAIN DNA COMPLEX

Template:ABSTRACT PUBMED 9813123

About this Structure

3HDD is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Engrailed homeodomain-DNA complex at 2.2 A resolution: a detailed view of the interface and comparison with other engrailed structures., Fraenkel E, Rould MA, Chambers KA, Pabo CO, J Mol Biol. 1998 Nov 27;284(2):351-61. PMID:9813123

Page seeded by OCA on Thu Jul 3 12:48:39 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hdd"
Personal tools