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| - | [[Image:3ovw.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_3ovw| PDB=3ovw | SCENE= }} | | {{STRUCTURE_3ovw| PDB=3ovw | SCENE= }} |
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| - | '''ENDOGLUCANASE I NATIVE STRUCTURE'''
| + | ===ENDOGLUCANASE I NATIVE STRUCTURE=== |
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| - | ==Overview==
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| - | The mechanisms involved in the enzymatic degradation of cellulose are of great ecological and commercial importance. The breakdown of cellulose by fungal species is performed by a consortium of free enzymes, known as cellobiohydrolases and endoglucanases, which are found in many of the 57 glycosyl hydrolase families. The structure of the endoglucanase I (EG I), found in glycosyl hydrolase family 7, from the thermophilic fungus Fusarium oxysporum has been solved at 2.3 A resolution. In addition to the native enzyme, structures have also been determined with both the affinity label, 3,4-epoxybutyl beta-D-cellobioside, and the reaction product cellobiose. The affinity label is covalently bound, as expected, to the catalytic nucleophile, Glu197, with clear evidence for binding of both the R and S stereoisomers. Cellobiose is found bound to the -2 and -1 subsites of the enzyme. In marked contrast to the structure of EG I with a nonhydrolyzable thiosaccharide analog, which spanned the -2, -1, and +1 subsites and which had a skew-boat conformation for the -1 subsite sugar [Sulzenbacher, G., et al. (1996) Biochemistry 35, 15280-15287], the cellobiose complex shows no pyranoside ring distortion in the -1 subsite, implying that strain is induced primarily by the additional +1 subsite interactions and that the product is found, as expected, in its unstrained conformation. | + | The line below this paragraph, {{ABSTRACT_PUBMED_9153432}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9153432 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9153432}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Glycosyl hydrolase]] | | [[Category: Glycosyl hydrolase]] |
| | [[Category: Glycosylated protein]] | | [[Category: Glycosylated protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:08:37 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 12:58:38 2008'' |
Revision as of 09:58, 3 July 2008
Template:STRUCTURE 3ovw
ENDOGLUCANASE I NATIVE STRUCTURE
Template:ABSTRACT PUBMED 9153432
About this Structure
3OVW is a Single protein structure of sequence from Fusarium oxysporum. Full crystallographic information is available from OCA.
Reference
Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution., Sulzenbacher G, Schulein M, Davies GJ, Biochemistry. 1997 May 13;36(19):5902-11. PMID:9153432
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