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421p

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{{STRUCTURE_421p| PDB=421p | SCENE= }}
{{STRUCTURE_421p| PDB=421p | SCENE= }}
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'''THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES'''
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===THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES===
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==Overview==
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The X-ray structures of the guanine nucleotide binding domains (amino acids 1-166) of five mutants of the H-ras oncogene product p21 were determined. The mutations described are Gly-12----Arg, Gly-12----Val, Gln-61----His, Gln-61----Leu, which are all oncogenic, and the effector region mutant Asp-38----Glu. The resolutions of the crystal structures range from 2.0 to 2.6 A. Cellular and mutant p21 proteins are almost identical, and the only significant differences are seen in loop L4 and in the vicinity of the gamma-phosphate. For the Gly-12 mutants the larger side chains interfere with GTP binding and/or hydrolysis. Gln-61 in cellular p21 adopts a conformation where it is able to catalyze GTP hydrolysis. This conformation has not been found for the mutants of Gln-61. Furthermore, Leu-61 cannot activate the nucleophilic water because of the chemical nature of its side chain. The D38E mutation preserves its ability to bind GAP.
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{{ABSTRACT_PUBMED_2199064}}
==About this Structure==
==About this Structure==
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[[Category: Wittinghofer, A.]]
[[Category: Wittinghofer, A.]]
[[Category: Oncogene protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:17:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:17:17 2008''

Revision as of 10:17, 3 July 2008

Image:421p.png

Template:STRUCTURE 421p

THREE-DIMENSIONAL STRUCTURES OF H-RAS P21 MUTANTS: MOLECULAR BASIS FOR THEIR INABILITY TO FUNCTION AS SIGNAL SWITCH MOLECULES

Template:ABSTRACT PUBMED 2199064

About this Structure

421P is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Three-dimensional structures of H-ras p21 mutants: molecular basis for their inability to function as signal switch molecules., Krengel U, Schlichting I, Scherer A, Schumann R, Frech M, John J, Kabsch W, Pai EF, Wittinghofer A, Cell. 1990 Aug 10;62(3):539-48. PMID:2199064

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