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422d

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{{STRUCTURE_422d| PDB=422d | SCENE= }}
{{STRUCTURE_422d| PDB=422d | SCENE= }}
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'''5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3''''
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===5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'===
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==Overview==
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The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.
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(as it appears on PubMed at http://www.pubmed.gov), where 14684886 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14684886}}
==About this Structure==
==About this Structure==
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[[Category: Sundaralingam, M.]]
[[Category: Sundaralingam, M.]]
[[Category: Double helix]]
[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:17:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:17:26 2008''

Revision as of 10:17, 3 July 2008

Image:422d.png

Template:STRUCTURE 422d

5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'

Template:ABSTRACT PUBMED 14684886

About this Structure

Full crystallographic information is available from OCA.

Reference

Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides., Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:14684886

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