464d

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{{STRUCTURE_464d| PDB=464d | SCENE= }}
{{STRUCTURE_464d| PDB=464d | SCENE= }}
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'''DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX'''
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===DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX===
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==Overview==
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An RNA helix with seven base pairs which was derived from the acceptor stem of Escherichia coli tRNA(Ala), rGGGGCUA.rUAGCUCC (ALA(wt)), as well as a variant, rGGGGCUA.rUAGCCCC (ALA(C70)), in which the single G.U wobble base pair of ALA(wt) was replaced by G.C, crystallize in space group C2. Both non-isomorphic crystal forms display a complex packing pattern, which can be described alternatively as disorder or pseudo-merohedral twinning. The structure of ALA(wt) was determined by SIRAS phasing using an isomorphous iodine derivative, rGGGGCi(5)UA.rUAGCUCC (ALA(I)). All three RNA structures were subsequently subjected to twin refinement in space group P1, using anisotropic thermal displacement parameters at resolutions of 1.16, 1.23 and 1.4 A for ALA(wt), ALA(I) and ALA(C70), respectively. Alternatively, the structure of ALA(wt) was refined in space group C2 assuming twofold disorder of the molecular orientation. The refined structures are of reasonable quality according to all available indicators. There are no systematic differences between the molecular models resulting from twin refinement and disorder refinement.
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(as it appears on PubMed at http://www.pubmed.gov), where 10417408 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10417408}}
==About this Structure==
==About this Structure==
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[[Category: Double helix]]
[[Category: Double helix]]
[[Category: Ribonucleic acid]]
[[Category: Ribonucleic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:23:29 2008''

Revision as of 10:23, 3 July 2008

Image:464d.png

Template:STRUCTURE 464d

DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX

Template:ABSTRACT PUBMED 10417408

About this Structure

Full crystallographic information is available from OCA.

Reference

Disorder and twin refinement of RNA heptamer double helices., Mueller U, Muller YA, Herbst-Irmer R, Sprinzl M, Heinemann U, Acta Crystallogr D Biol Crystallogr. 1999 Aug;55(Pt 8):1405-13. PMID:10417408

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