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4bna

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{{STRUCTURE_4bna| PDB=4bna | SCENE= }}
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'''REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER: CGCGAATTBRCGCG'''
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===REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER: CGCGAATTBRCGCG===
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==Overview==
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A double-helical B-DNA dodecamer has been analyzed by single crystal x-ray diffraction methods and refined independently in four variants: sequence CGCGAATTCGCG at 20 degrees C and at 16 K, and CGCGAATTBrCGCG in 60% methylpentanediol at 20 and at 7 degrees C. The first three forms show a 14-19 degrees bend in overall helix axis, but the fourth is straight and unbent. Detailed comparisons of the various forms have led to a better understanding of helix geometry and bending. Structural principles can be understood best if organized under four headings: 1) intrinsic geometry of the sugar rings, 2) stacking and relative motion of base pairs, 3) geometry of the connecting phosphate backbone, and 4) mechanics of bending in B-DNA. The observed bending is neither completely localized nor smooth and continuous, but an intermediate compromise that can be termed "annealed kinking."
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(as it appears on PubMed at http://www.pubmed.gov), where 7174662 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7174662}}
==About this Structure==
==About this Structure==
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[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:30:15 2008''

Revision as of 10:30, 3 July 2008

Image:4bna.png

Template:STRUCTURE 4bna

REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER: CGCGAATTBRCGCG

Template:ABSTRACT PUBMED 7174662

About this Structure

Full crystallographic information is available from OCA.

Reference

Reversible bending and helix geometry in a B-DNA dodecamer: CGCGAATTBrCGCG., Fratini AV, Kopka ML, Drew HR, Dickerson RE, J Biol Chem. 1982 Dec 25;257(24):14686-707. PMID:7174662

Page seeded by OCA on Thu Jul 3 13:30:15 2008

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