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| - | [[Image:4eng.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_4eng| PDB=4eng | SCENE= }} | | {{STRUCTURE_4eng| PDB=4eng | SCENE= }} |
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| - | '''STRUCTURE OF ENDOGLUCANASE V CELLOHEXAOSE COMPLEX'''
| + | ===STRUCTURE OF ENDOGLUCANASE V CELLOHEXAOSE COMPLEX=== |
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| - | ==Overview==
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| - | The structure of the catalytic core of the endoglucanase V (EGV) from Humicola insolens has been determined by the method of multiple isomorphous replacement at 1.5 A resolution. The final model, refined with X-PLOR and PROLSQ, has a crystallographic R factor of 0.163 (R(free) = 0.240) with deviations from stereochemical target values of 0.012 A and 0.037 degrees for bonds and angles, respectively. The model was further refined with SHELXL, including anisotropic modelling of the protein-atom temperature factors, to give a final model with an R factor of 0.105 and an R(free) of 0.154. The initial isomorphous replacement electron-density map was poor and uninterpretable but was improved by the use of synchrotron data collected at a wavelength chosen so as to optimize the f" contribution of the anomalous scattering from the heavy atoms. The structure of H. insolens EGV consists of a six-stranded beta-barrel domain, similar to that found in a family of plant defence proteins, linked by a number of disulfide-bonded loop regions. A long open groove runs across the surface of the enzyme either side of which lie the catalytic aspartate residues. The 9 A separation of the catalytic carboxylate groups is consistent with the observation that EGV catalyzes the hydrolysis of the cellulose, beta(1-->4) links with inversion of configuration at the anomeric C1 atom. This structure is the first representative from the glycosyl hydrolase family 45. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15299721}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15299721 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15299721}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Glycosyl hydrolase]] | | [[Category: Glycosyl hydrolase]] |
| | [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:23:16 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:35:31 2008'' |
Revision as of 10:35, 3 July 2008
Template:STRUCTURE 4eng
STRUCTURE OF ENDOGLUCANASE V CELLOHEXAOSE COMPLEX
Template:ABSTRACT PUBMED 15299721
About this Structure
4ENG is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.
Reference
Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5 A resolution., Davies GJ, Dodson G, Moore MH, Tolley SP, Dauter Z, Wilson KS, Rasmussen G, Schulein M, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):7-17. PMID:15299721
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