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4gsp

From Proteopedia

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[[Image:4gsp.jpg|left|200px]]
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{{STRUCTURE_4gsp| PDB=4gsp | SCENE= }}
{{STRUCTURE_4gsp| PDB=4gsp | SCENE= }}
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'''RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS'''
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===RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS===
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==Overview==
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Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.
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(as it appears on PubMed at http://www.pubmed.gov), where 9546218 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9546218}}
==About this Structure==
==About this Structure==
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[[Category: Endoribonuclease]]
[[Category: Endoribonuclease]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:24:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:39:35 2008''

Revision as of 10:39, 3 July 2008

Image:4gsp.png

Template:STRUCTURE 4gsp

RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 7 DAYS

Template:ABSTRACT PUBMED 9546218

About this Structure

4GSP is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218

Page seeded by OCA on Thu Jul 3 13:39:35 2008

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