4ovw

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[[Image:4ovw.jpg|left|200px]]
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{{STRUCTURE_4ovw| PDB=4ovw | SCENE= }}
{{STRUCTURE_4ovw| PDB=4ovw | SCENE= }}
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'''ENDOGLUCANASE I COMPLEXED WITH EPOXYBUTYL CELLOBIOSE'''
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===ENDOGLUCANASE I COMPLEXED WITH EPOXYBUTYL CELLOBIOSE===
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==Overview==
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The mechanisms involved in the enzymatic degradation of cellulose are of great ecological and commercial importance. The breakdown of cellulose by fungal species is performed by a consortium of free enzymes, known as cellobiohydrolases and endoglucanases, which are found in many of the 57 glycosyl hydrolase families. The structure of the endoglucanase I (EG I), found in glycosyl hydrolase family 7, from the thermophilic fungus Fusarium oxysporum has been solved at 2.3 A resolution. In addition to the native enzyme, structures have also been determined with both the affinity label, 3,4-epoxybutyl beta-D-cellobioside, and the reaction product cellobiose. The affinity label is covalently bound, as expected, to the catalytic nucleophile, Glu197, with clear evidence for binding of both the R and S stereoisomers. Cellobiose is found bound to the -2 and -1 subsites of the enzyme. In marked contrast to the structure of EG I with a nonhydrolyzable thiosaccharide analog, which spanned the -2, -1, and +1 subsites and which had a skew-boat conformation for the -1 subsite sugar [Sulzenbacher, G., et al. (1996) Biochemistry 35, 15280-15287], the cellobiose complex shows no pyranoside ring distortion in the -1 subsite, implying that strain is induced primarily by the additional +1 subsite interactions and that the product is found, as expected, in its unstrained conformation.
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(as it appears on PubMed at http://www.pubmed.gov), where 9153432 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9153432}}
==About this Structure==
==About this Structure==
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[[Category: Glycosyl hydrolase]]
[[Category: Glycosyl hydrolase]]
[[Category: Glycosylated protein]]
[[Category: Glycosylated protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:45:51 2008''

Revision as of 10:45, 3 July 2008

Image:4ovw.png

Template:STRUCTURE 4ovw

ENDOGLUCANASE I COMPLEXED WITH EPOXYBUTYL CELLOBIOSE

Template:ABSTRACT PUBMED 9153432

About this Structure

4OVW is a Single protein structure of sequence from Fusarium oxysporum. Full crystallographic information is available from OCA.

Reference

Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution., Sulzenbacher G, Schulein M, Davies GJ, Biochemistry. 1997 May 13;36(19):5902-11. PMID:9153432

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